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Although aging plays a major role in wound healing, it is often neglected in preclinical rodent models and clinical testing, resulting in a limited understanding of the mechanisms by which age slows healing (Gould et al., 2015)

Bacillus Coagulans support the microbiota bacteria, which are part of the microbiome, as well as the genetic material and metabolites associated with these bacteria

After centrifugation and thorough removal of the supernatant, the pellets were incubated for 1 h in 60 l staining solution (1% BSA and 0.5% Tween-20 in PBS) containing 3 l anti-BrdUFITC monoclonal antibody (BioLegend, 364104, clone 3D4)